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Elchrom Scientific horizontal sea2000 apparatus
Horizontal Sea2000 Apparatus, supplied by Elchrom Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/horizontal+sea2000+apparatus/sea+2000++advanced+submerged+gel+electrophoresis+apparatus/pm33432353-70-38-41
Average 90 stars, based on 1 article reviews
horizontal sea2000 apparatus - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Blocking Assay:

Article Title: Genetic variation in the mitochondrial cytochrome <i>c</i> oxidase subunit 1 within <i>Progamotaenia festiva</i> (Cestoda: Anoplocephalidae) from macropodid marsupials
Article Snippet: Genetic variation was examined in the anoplocephalid cestode Progamotaenia festiva, from Australian marsupials, in order to test the hypothesis that P. festiva, is a complex of sibling species and to assess the extent of host switching reported previously based on multilocus enzyme electrophoresis (MEE).. Polymerase chain reaction (PCR)-based single-strand conformational polymorphism (SSCP)was used for the analysis of sequence variation in the cytochrome c oxidase subunit 1 (cox1) gene among 179 specimens of P. festiva (identified based on morphology and predilection site in the host) from 13 different host species, followed by selective DNA sequencing.. Fifty-three distinct sequence types (haplotypes) representing all specimens were defined.

Article Title: Genetic variation in the mitochondrial cytochrome c oxidase subunit 1 within three species of Progamotaenia (Cestoda: Anoplocephalidae) from macropodid marsupials.
Article Snippet: Sequence variation within 3morphologically defined species of the anoplocephalid cestode genusProgamotaenia (P. ewersi, P. macropodis and P. zschokkei) was investigated using the cytochrome c oxidase subunit 1 gene.. The magnitude of genetic variation detected within each morphospecies suggests that, in each instance, several cryptic species are present.. Within P. ewersi, 5 genetically distict groups of cestodes were detected, 1 shared byMacropus robustus andM. parryi in Queensland, 1 in M. agilis from Queensland, 1 in Petrogale assimilis from Queensland, 1 in Macropus fuliginosus from South Australia and 1 in Wallabia bicolor from Victoria.

Article Title: Genetic variants of Malassezia pachydermatis from canine skin: body distribution and phospholipase activity.
Article Snippet: .. In brief, after denaturation at 94 1C for 30 min and snap cooling on a freeze block ( 20 1C), 15 mL of each sample (i.e. 1–2mL of amplicon plus 8 mL of water plus 5 mL of loading buffer) were loaded into the wells of precast Gene Mutation Analysis (GMA) gels (S-2 13, Elchrom Scientific AG) and subjected to electrophoresis for 19 h at 74 V and 7.4 1C (constant) in a horizontal SEA2000 apparatus (Elchrom Scientific) connected to a MultiTemp III (Pharmacia) cooling system. .. After electrophoresis, gels were stained for 15 min with SYBR Gold (1 : 10 000 dilution) and photographed using a GelDoc system (Bio-Rad).

Electrophoresis:

Article Title: Genetic variation in the mitochondrial cytochrome <i>c</i> oxidase subunit 1 within <i>Progamotaenia festiva</i> (Cestoda: Anoplocephalidae) from macropodid marsupials
Article Snippet: Genetic variation was examined in the anoplocephalid cestode Progamotaenia festiva, from Australian marsupials, in order to test the hypothesis that P. festiva, is a complex of sibling species and to assess the extent of host switching reported previously based on multilocus enzyme electrophoresis (MEE).. Polymerase chain reaction (PCR)-based single-strand conformational polymorphism (SSCP)was used for the analysis of sequence variation in the cytochrome c oxidase subunit 1 (cox1) gene among 179 specimens of P. festiva (identified based on morphology and predilection site in the host) from 13 different host species, followed by selective DNA sequencing.. Fifty-three distinct sequence types (haplotypes) representing all specimens were defined.

Article Title: Genetic variation in the mitochondrial cytochrome c oxidase subunit 1 within three species of Progamotaenia (Cestoda: Anoplocephalidae) from macropodid marsupials.
Article Snippet: Sequence variation within 3morphologically defined species of the anoplocephalid cestode genusProgamotaenia (P. ewersi, P. macropodis and P. zschokkei) was investigated using the cytochrome c oxidase subunit 1 gene.. The magnitude of genetic variation detected within each morphospecies suggests that, in each instance, several cryptic species are present.. Within P. ewersi, 5 genetically distict groups of cestodes were detected, 1 shared byMacropus robustus andM. parryi in Queensland, 1 in M. agilis from Queensland, 1 in Petrogale assimilis from Queensland, 1 in Macropus fuliginosus from South Australia and 1 in Wallabia bicolor from Victoria.

Article Title: Genetic variation in the 16S mitochondrial DNA gene of two Canadian populations of Dermacentor andersoni (Acari: Ixodidae).
Article Snippet: The Rocky Mountain wood tick, Dermacentor andersoni Stiles, 1908, is of medical and veterinary importance because it can transmit pathogenic agents to humans, domestic livestock, and wildlife.. The preferred attachment sites of D. andersoni adults and their ability to induce paralysis in hosts vary among populations, which may have a genetic basis.. In this study, polymerase chain reaction (PCR)Ðsingle-strand conformation polymorphism (SSCP) analyses and DNA sequencing were used to determine the genetic variation in the 16S mitochondrial DNA gene of twoD. andersonipopulations from the Canadian prairies: Saskatchewan Landing Provincial Park, Saskatchewan, and Lethbridge, Alberta.

Article Title: Genetic variants of Malassezia pachydermatis from canine skin: body distribution and phospholipase activity.
Article Snippet: .. In brief, after denaturation at 94 1C for 30 min and snap cooling on a freeze block ( 20 1C), 15 mL of each sample (i.e. 1–2mL of amplicon plus 8 mL of water plus 5 mL of loading buffer) were loaded into the wells of precast Gene Mutation Analysis (GMA) gels (S-2 13, Elchrom Scientific AG) and subjected to electrophoresis for 19 h at 74 V and 7.4 1C (constant) in a horizontal SEA2000 apparatus (Elchrom Scientific) connected to a MultiTemp III (Pharmacia) cooling system. .. After electrophoresis, gels were stained for 15 min with SYBR Gold (1 : 10 000 dilution) and photographed using a GelDoc system (Bio-Rad).

Transferring:

Article Title: Single-strand conformation polymorphism (SSCP) for the analysis of genetic variation.
Article Snippet: 1Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030, Australia.. 2Department of Biology, University of Saskatchewan, Saskatchewan, Canada, S7N 5E2.. 3Dipartimento di Sanità e Benessere Animale, Facoltà di Medicina Veterinaria, Valenzano, Bari 70010, Italy.

Polymerase Chain Reaction:

Article Title: Single-strand conformation polymorphism (SSCP) for the analysis of genetic variation.
Article Snippet: 1Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030, Australia.. 2Department of Biology, University of Saskatchewan, Saskatchewan, Canada, S7N 5E2.. 3Dipartimento di Sanità e Benessere Animale, Facoltà di Medicina Veterinaria, Valenzano, Bari 70010, Italy.

Agarose Gel Electrophoresis:

Article Title: Single-strand conformation polymorphism (SSCP) for the analysis of genetic variation.
Article Snippet: 1Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030, Australia.. 2Department of Biology, University of Saskatchewan, Saskatchewan, Canada, S7N 5E2.. 3Dipartimento di Sanità e Benessere Animale, Facoltà di Medicina Veterinaria, Valenzano, Bari 70010, Italy.

Sequencing:

Article Title: Single-strand conformation polymorphism (SSCP) for the analysis of genetic variation.
Article Snippet: 1Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030, Australia.. 2Department of Biology, University of Saskatchewan, Saskatchewan, Canada, S7N 5E2.. 3Dipartimento di Sanità e Benessere Animale, Facoltà di Medicina Veterinaria, Valenzano, Bari 70010, Italy.

Nucleic Acid Electrophoresis:

Article Title: Single-strand conformation polymorphism (SSCP) for the analysis of genetic variation.
Article Snippet: 1Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030, Australia.. 2Department of Biology, University of Saskatchewan, Saskatchewan, Canada, S7N 5E2.. 3Dipartimento di Sanità e Benessere Animale, Facoltà di Medicina Veterinaria, Valenzano, Bari 70010, Italy.

Labeling:

Article Title: Single-strand conformation polymorphism (SSCP) for the analysis of genetic variation.
Article Snippet: 1Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030, Australia.. 2Department of Biology, University of Saskatchewan, Saskatchewan, Canada, S7N 5E2.. 3Dipartimento di Sanità e Benessere Animale, Facoltà di Medicina Veterinaria, Valenzano, Bari 70010, Italy.

DNA Extraction:

Article Title: Single-strand conformation polymorphism (SSCP) for the analysis of genetic variation.
Article Snippet: 1Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030, Australia.. 2Department of Biology, University of Saskatchewan, Saskatchewan, Canada, S7N 5E2.. 3Dipartimento di Sanità e Benessere Animale, Facoltà di Medicina Veterinaria, Valenzano, Bari 70010, Italy.

Amplification:

Article Title: Genetic variation in the 16S mitochondrial DNA gene of two Canadian populations of Dermacentor andersoni (Acari: Ixodidae).
Article Snippet: The Rocky Mountain wood tick, Dermacentor andersoni Stiles, 1908, is of medical and veterinary importance because it can transmit pathogenic agents to humans, domestic livestock, and wildlife.. The preferred attachment sites of D. andersoni adults and their ability to induce paralysis in hosts vary among populations, which may have a genetic basis.. In this study, polymerase chain reaction (PCR)Ðsingle-strand conformation polymorphism (SSCP) analyses and DNA sequencing were used to determine the genetic variation in the 16S mitochondrial DNA gene of twoD. andersonipopulations from the Canadian prairies: Saskatchewan Landing Provincial Park, Saskatchewan, and Lethbridge, Alberta.

Article Title: Genetic variants of Malassezia pachydermatis from canine skin: body distribution and phospholipase activity.
Article Snippet: .. In brief, after denaturation at 94 1C for 30 min and snap cooling on a freeze block ( 20 1C), 15 mL of each sample (i.e. 1–2mL of amplicon plus 8 mL of water plus 5 mL of loading buffer) were loaded into the wells of precast Gene Mutation Analysis (GMA) gels (S-2 13, Elchrom Scientific AG) and subjected to electrophoresis for 19 h at 74 V and 7.4 1C (constant) in a horizontal SEA2000 apparatus (Elchrom Scientific) connected to a MultiTemp III (Pharmacia) cooling system. .. After electrophoresis, gels were stained for 15 min with SYBR Gold (1 : 10 000 dilution) and photographed using a GelDoc system (Bio-Rad).

Mutagenesis:

Article Title: Genetic variants of Malassezia pachydermatis from canine skin: body distribution and phospholipase activity.
Article Snippet: .. In brief, after denaturation at 94 1C for 30 min and snap cooling on a freeze block ( 20 1C), 15 mL of each sample (i.e. 1–2mL of amplicon plus 8 mL of water plus 5 mL of loading buffer) were loaded into the wells of precast Gene Mutation Analysis (GMA) gels (S-2 13, Elchrom Scientific AG) and subjected to electrophoresis for 19 h at 74 V and 7.4 1C (constant) in a horizontal SEA2000 apparatus (Elchrom Scientific) connected to a MultiTemp III (Pharmacia) cooling system. .. After electrophoresis, gels were stained for 15 min with SYBR Gold (1 : 10 000 dilution) and photographed using a GelDoc system (Bio-Rad).



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